The research group's work had finally gotten on track, and the atmosphere in the office had shifted from the initial adrenaline rush to a more sustained calm.
The five PhDs were learning theory while starting to get hands-on, and the tedium and challenges of scientific research were gradually beginning to reveal themselves.
Liu Yang's molecular cloning platform encountered its first hurdle—after synthesizing the first batch of enhancer gene fragments he designed, the Golden Gate assembly efficiency was dismally low, with those enzymes and DNA fragments stubbornly refusing to fit together according to his blueprint on the microplate.
Staring at the sparse bands on the electrophoresis gel, he frowned deeply.
"It shouldn't be like this. I've optimized the ligation temperature, enzyme concentration, and fragment ratio!" Liu Yang scratched his already messy hair.
