He suddenly shifted his tone and proposed a more radical idea: "At the same time, I support our efforts to explore brand new connection methods. I have noticed that in recent years, there is a split-intein system modified based on the principle of 'protein splicing', which theoretically can achieve seamless connection of the C-terminus and N-terminus of proteins. If we can modify its recognition sequences and reaction conditions to connect two independent proteins, it will be a revolutionary breakthrough. But this requires a lot of protein engineering modifications, and it's extremely difficult."
Yang Ping gently tapped the desktop with his fingers, pondering for a moment: "The idea is clear. Just as you said, at this stage, focus on the SpyTag/SnoopTag system and ensure we quickly produce proof-of-concept data. Meanwhile, you pursue exploration of the split-intein and other possible new methods. If you need any special expert support in this aspect, just tell me directly."
